Optimisation of pre-harvest sago frond sap for the production of l-lactic acid using Lactococcus lactis IO-1

The major restriction of sago palm plantation is the long maturation period for starch extraction. As such, alternative commodity has to be identified increase commercial value especially from by-product. Sago frond been harvested have numerous advantages as an substrate production biomaterial and biofuel such lactic acid bioethanol. This study focuses on effective approach maximise concentration sugar in identify ideal sap (SFSp) sole media produce l-lactic grow L. lactis IO-1. Result shows that SFSp extracted rachis using a roller press machine contained glucose (43.8 g/L), xylose (17.3 g/L) free sugars (5.55 g/L). Combination enzymatic hydrolysis sterilisation pre-treatment was found total carbon source (68 sterilised suitable used fermentation process. determined at 50% dilution produces highest amount (24.02 synergist by most efficient consumption (89.93%). use medium generated maximum biomass 14.53 g/L. utilisation pre-harvest promote efficiency associated exceptional growth performance In conclusion, concept utilizing by-product novel raw material promise reliable resources superiority IO-1 metabolise lucrative ethical acid.